The plastic horns, popular with football fans at the 2010 World Cup in South Africa, have already been banned by some Football League clubs.
In a statement, Spurs said the decision had been made after undergoing discussions with the police and the local licensing authorities.
They club also suggested the noise could pose risks to public safety. "We are concerned that the presence of the instruments within the stadium pose unnecessary risks and could impact on the ability of all supporters to hear any emergency safety announcements," read the statement on the
club's official website. "We are very proud of the fantastic atmosphere that our supporters produce organically at White Hart Lane and we are all very much looking forward to this continuing into the forthcoming season."
The Premier League has refused to implement a widespread ban, stating that "such matters are dealt with at club level".
Following their widespread use during the World Cup in South Africa, retailers across Britain have being selling vuvuzelas in anticipation of their popularity.
However, the horns have been heavily criticised by players and fans alike with many suggesting they are tuneless and block out singing and chanting.